The Effect of Neonatal Leptin Antagonism in Male Rat Offspring Is Dependent upon the Interaction between Prior Maternal Nutritional Status and Post-Weaning Diet

Table 1

Adult (P110) body weight, total fat (%), body length, bone density, and mineral content in CON or MHF offspring treated with either saline (S) or pegylated leptin antagonist (LA) as neonates and fed either a chow or hf diet after weaning. Data are means ± SEM, 𝑛=8–16 per group.

Group	Final body weight (g)	Total body Fat (%)	Lean mass (%)	Fat : lean ratio	Nose-anus (mm)	Nose-tail (mm)	BMD (g/cm^2)	BMC (g)
CON-S-chow	590 ± 10	31.0 ± 2.3	67.2 ± 2.2	0.47 ± 0.05	277 ± 2	478 ± 2	0.174 ± 0.001	14.8 ± 0.4
CON-S-hf	723 ± 19	46.6 ± 2.6	52.0 ± 2.5	0.91± 0.09	279 ± 2	484 ± 4	0.183 ± 0.003	17.6 ± 0.6
CON-LA-chow	595 ± 17	29.1 ± 1.1	69.3 ± 0.9	0.43 ± 0.02	277 ± 3	479 ± 4	0.171 ± 0.002	14.5 ± 0.2
CON-LA-hf	650 ± 21	37.9 ± 3.1	60.0 ± 3.8	0.72 ± 0.09	278 ± 2	489 ± 3	0.174 ± 0.003	15.8 ± 0.6
MHF-S-chow	621 ± 11	38.9 ± 2.1	59.6 ± 2.1	0.65 ± 0.06	277 ± 2	477 ± 3	0.176 ± 0.002	15.7 ± 0.3
MHF-S-hf	729 ± 18	51.0 ± 1.6	47.8 ± 1.5	1.06 ± 0.07	280 ± 2	480 ± 3	0.187 ± 0.003	18.7 ± 0.4
MHF-LA-chow	624 ± 13	34.9 ± 1.5	63.5 ± 1.5	0.54 ± 0.03	283 ± 1	490 ± 2	0.171 ± 0.001	14.8 ± 0.3
MHF-LA-hf	710 ± 18	44.1 ± 3.0	50.8 ± 1.9	0.94 ± 0.08	287 ± 2	493 ± 4	0.179 ± 0.002	17.5 ± 0.4
Main effects
Maternal diet (MD)	𝑃<0.05	𝑃<0.005	𝑃<0.0001	𝑃<0.005	𝑃<0.05	NS	NS	𝑃<0.005
LA treatment (LA)	NS	𝑃<0.005	𝑃<0.05	𝑃<0.05	𝑃<0.05	𝑃<0.005	𝑃<0.0001	𝑃<0.005
PW-diet	𝑃<0.0001	𝑃<0.0001	𝑃<0.0001	𝑃<0.0001	NS	𝑃<0.05	𝑃<0.0001	𝑃<0.0001
Interactions
MD × LA	NS	NS	NS	NS	𝑃<0.05	𝑃<0.05	NS	NS
LA × PW-diet	𝑃=0.05	NS	NS	NS	NS	NS	NS	NS
MD × PW diet	NS	NS	NS	NS	NS	NS	NS	NS
MD × PW diet × LA	NS	NS	NS	NS	NS	NS	NS	NS