Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats
Figure 3
qRT-PCR analysis of genes for (a) Cdc2, (b) Ccnb1, (c) Top2a, (d) Hmgcs2, and (e) Aqp8 in the distal colonic mucosa of rats fed either an AIN (control), black bean- (BB-), or soy flour- (SF-) based diet. Results were normalized to the housekeeping gene β-Actin and are presented as relative fold-changes (LSM ± SEM) standardized to the AIN (control) diet. *Denotes significance compared to AIN controls; +denotes significance between BB and SF-fed animals (). Abbreviations: Cdc2, cell division cycle 2; Ccnb1, cyclin B1; Top2a, topoisomerase II alpha; Hmgcs2, 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2; Aqp8, Aquaporin 8.