Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats

Table 3

Genes similarly affected by bean-feeding (BB and SF) in the distal colonic epithelium of male F344 rats¹.

Gene symbol	Gene title	BB	SF
(I) Cell cycle, cell growth and maintenance, apoptosis
Ceacam1	carcinoembryonic antigen-related cell adhesion molecule 1	2.3*	1.9*
Rb1	retinoblastoma 1	2.2*	2.2*
Gadd45a	growth arrest and DNA-damage-inducible, alpha	1.5*	1.5*
Bax	Bcl2-associated X protein	1.3	1.4*
Egln3	EGL nine homolog 3 (C. elegans)	0.67*	0.79
Wfdc1	WAP four-disulfide core domain 1	0.67*	0.50*
Ccnb1	cyclin B1	0.66*	0.85
Rfc4	replication factor C (activator 1) 4	0.59*	1.1
Cdc2	cell division cycle 2, G1 to S and G2 to M	0.58*	0.83
Top2a	topoisomerase (DNA) II alpha	0.57*	0.85
Bub1b	budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae)	0.49*	0.60*
(II) Channel, transporters, & carriers
Aqp8	aquaporin 8	2.7*	2.9*
Scnn1g	sodium channel, nonvoltage-gated 1 gamma	2.2*	2.0*
Apoa1	apolipoprotein A-I	1.6	2.4*
Slc12a7	solute carrier family 12 (potassium/chloride transporters), member 7	1.6*	1.4*
Slc5a1	solute carrier family 5 (sodium/glucose cotransporter), member 1	1.5*	1.3
Slc16a1	solute carrier family 16, member 1 (monocarboxylic acid transporter 1)	1.5*	1.5*
Atp1a1	ATPase, Na^+/K^+ transporting, alpha 1 polypeptide	1.4*	1.3*
Lgals9	lectin, galactoside-binding, soluble, 9	1.3*	1.3*
Kcnk1	potassium channel, subfamily K, member 1	1.3*	1.3*
Slco1a1	solute carrier organic anion transporter family, member 1a1	0.68	0.35*
SLC16A6	solute carrier family 16, member 6 (monocarboxylic acid transporter 7)	0.61*	0.82
Fabp5	fatty acid binding protein 5, epidermal	0.48*	0.64*
Ttpa	tocopherol (alpha) transfer protein	0.47*	0.66*
(III) Electron transport, oxidoreductase, detoxification
Cyp4b1	cytochrome P450, family 4, subfamily b, polypeptide 1	3.6*	2.2*
Gstm5	glutathione S-transferase, mu 5	1.5*	1.5*
Gstm1	glutathione S-transferase mu 1	1.4*	1.3
Cyp27a1	cytochrome P450, family 27, subfamily a, polypeptide 1	1.4*	1.5*
Prdx6	peroxiredoxin 6	1.4	1.5*
Cyp2d1	cytochrome P450, family 2, subfamily d, polypeptide 1	1.4*	1.4*
Por	P450 (cytochrome) oxidoreductase	1.4*	1.4*
Cyb5a	cytochrome b5 type A (microsomal)	1.4*	1.3*
Tst	thiosulfate sulfurtransferase, mitochondrial	1.3*	1.3*
(IV) Energy metabolism
Hmgcs2	3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2	2.2*	2.1*
Aldob	aldolase B, fructose-bisphosphate	2.1*	1.8*
Pck1	phosphoenolpyruvate carboxykinase 1 (soluble)	2.0*	1.8*
Ech1	enoyl coenzyme A hydratase 1, peroxisomal	1.6*	1.6*
Glul	glutamate-ammonia ligase	1.4*	1.2*
Hadhb	hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit	1.3*	1.3*
Cbr1	carbonyl reductase 1	1.2	1.5*
Hadha	hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit	1.2*	1.3*
Pfkp	phosphofructokinase, platelet	0.67*	0.76*
Gpd2	glycerol-3-phosphate dehydrogenase 2, mitochondrial	0.67*	0.82
Acsl4	acyl-CoA synthetase long-chain family member 4	0.64*	0.80
Pyy	peptide YY	0.64*	0.72*
Gcg	glucagon	0.57*	0.81
Scd	stearoyl-CoA desaturase (delta-9-desaturase)	0.53*	0.72
Scd1	stearoyl-Coenzyme A desaturase 1	0.47*	0.65
(V) Extracellular matrix, cell adhesion, cytoskeleton
Sdc1	syndecan 1	1.3*	1.3*
Sparc	secreted protein, acidic, cysteine-rich (osteonectin)	1.0	0.48*
Fn1	fibronectin 1	0.66*	0.61*
Tubb5	tubulin, beta 5	0.65*	0.92
Col1a1	collagen, type I, alpha 1	0.53*	0.52*
(VI) Immune, defense, inflammation, stress
Hspa1a	heat shock 70 kD protein 1A	1.9*	1.4
RT1-EC2	RT1 class Ib, locus Aw2	1.5*	1.4*
Tlr4	toll-like receptor 4	0.78*	0.68*
Mif	macrophage migration inhibitory factor	0.71*	0.88
Dmbt1	deleted in malignant brain tumors 1	0.64*	0.74*
Pla2g2a	phospholipase A2, group IIA (platelets, synovial fluid)	0.50*	0.39*
RatNP-3	defensin RatNP-3 precursor	0.48*	0.69*
Cxcl14	chemokine (C-X-C motif) ligand 14	0.41*	0.48*
(VII) Nucleic acid binding, transcription regulation
Nr1d2	nuclear receptor subfamily 1, group D, member 2	2.2*	1.3
Nfib	nuclear factor I/B	1.5*	1.4*
Vdr	vitamin D (1,25- dihydroxyvitamin D3) receptor	1.4	1.6*
Syt4	synaptotagmin IV	0.82	0.65*
Egr2	early growth response 2	0.77*	0.60*
Nr4a2	nuclear receptor subfamily 4, group A, member 2	0.70*	0.75*
Egr1	early growth response 1	0.50*	0.66*
(VIII) Signal transduction
Gchfr	GTP cyclohydrolase I feedback regulator	1.9*	1.8*
Ppm1b	protein phosphatase 1B, magnesium dependent, beta isoform	1.6*	1.4
Mapk14	mitogen activated protein kinase 14	1.3*	1.4*
Gucy2c	guanylate cyclase 2C	1.3*	1.4*
P2ry2	purinergic receptor P2Y, G-protein coupled 2	0.81	0.61*
Ptpn3	protein tyrosine phosphatase, non-receptor type 3	0.79*	0.73*
P2ry6	pyrimidinergic receptor P2Y, G-protein coupled, 6	0.69*	0.68*
Pld1	phospholipase D1	0.68*	0.85
Ptpn18	protein tyrosine phosphatase, non-receptor type 18	0.63*	0.83*
Ptpro	protein tyrosine phosphatase, receptor type, O	0.62*	0.72*
Fzd1	frizzled homolog 1 (Drosophila)	0.62*	0.74*

¹Data expressed as mean-fold change normalized to the AIN group (𝑛=8/group). *denotes significant difference compared to the control (AIN) diet (𝑃<0.05).