Plant Polyphenols and Oxidative Metabolites of the Herbal Alkenylbenzene Methyleugenol Suppress Histone Deacetylase Activity in Human Colon Carcinoma Cells
Figure 3
Inhibition of HDAC1 activity in HT29 cells after 24 h of incubation with (a) polyphenols in the presence of catalase (100 U/mL) or (b) methyleugenol and methyleugenol metabolites. HDAC activity was determined by the metabolic rate of a specific HDAC1 substrate. Recombinant HDAC1 was included as positive control (PC) and trichostatin A (TA) as a specific HDAC inhibitor. The data presented are mean ± SD of at least three independent experiments, each performed in a duplicate. The significances indicated were calculated in relation to the solvent control DMSO 0.5% v/v (Student’s -test: ; ).
