Leucine Modulates Mitochondrial Biogenesis and SIRT1-AMPK Signaling in C2C12 Myotubes

Figure 5

Leucine-induced mitochondrial biogenesis in C2C12 myotubes requires AMPK. (a) C2C12 myotubes were treated with leucine (0.5 mM), AICAR (20 μM), and Compound C (25 μM) for 24 hours. mtDNA levels of the cells were analyzed by the mitochondrial markers gene expression, Hspd1 and COX2, using real-time PCR. (b) Sirt1 and mitochondrial biogenesis related mRNA level of PGC-1α and COX5b were evaluated also by RT-PCR after treating with leucine and Compound C for 24 hours was measured; all the mRNA levels were normalized to 18S housekeeping gene. Data are mean ± SE (). Dark bars are vehicle control; grey bars are Compound C. Significantly different from controls. Significant Compound C effects.

(a)

(b)