Nutrition and Reproductive Health: Sperm versus Erythrocyte Lipidomic Profile and ω-3 Intake
Table 2
Fatty acid profile in erythrocyte membranes.
FAME
Subjects before supplementation (; % rel)
Subjects after supplementation (; % rel)
Optimal interval values (% rel)
value
16:0
26.8 (24.3; 29.8)
27.3 (24.1; 29)
17–27
0.8326
18:0
20.7 (19.1; 21.4)
19.5 (16.9; 21.5)
13–20
0.0294
9cis-16:1
0.4 (0.2; 0.6)
0.6 (0.4; 0.7)
0.2–0.5
0.04
9cis-18:1
15.8 (14.2; 18.3)
16.0 (14.3; 17.7)
9–18
0.2138
11cis-18:1
1.4 (1.1; 1.7)
1.3 (1; 1.6)
0.7–1.3
0.4072
18:2 (omega-6, LA)
10.3 (9.5; 14.3)
12.3 (10.0; 14.7)
9–16
0.0044
20:3 (omega-6, GLA)
2.0 (1.4; 3.4)
2.3 (1.3; 3.3)
1.9–2.4
0.863
20:4 (omega-6, ARA)
18.1 (16.6; 19.1)
15.9 (14.0; 19.2)
13–17
0.0092
20:5 (omega-3, EPA)
0.4 (0.2; 0.5)
0.9 (0.6; 1.2)
0.5–0.9
0.0112
22:6 (omega-3, DHA)
3.3 (2.2; 4.5)
4.3 (2.7; 6.3)
5–7
0.0366
Trans-18:1
0.2 (0.1; 0.3)
0.1 (0; 0.2)
0.1–0.3
0.0306
Total SFA
47.5 (45.0; 50.7)
46.9 (42.6; 48.5)
30–45
0.1636
Total MUFA
17.5 (16.1; 20.6)
18.1 (15.9; 19.7)
13–23
0.2856
Total PUFA
34.2 (32.4; 38.2)
35.5 (31.5; 39.8)
28–39
0.3868
SFA/MUFA
2.7 (2.3; 3.1)
2.5 (2.4; 3.0)
1.7–2
0.0196
Omega-6/omega-3
8.4 (6.2; 13.7)
5.7 ± (4.5; 9.4)
3.5–5.5
0.01
CV risk index
3.6 (2.6; 4.8)
5.2 (3.4; 7.2)
0–4%, high risk 4–8%, intermediate risk >8%, minimal risk
0.0098
The values of the fatty acids are reported as relative percentage (% rel) of the total of 12 fatty acids chosen as representative components of the erythrocyte membrane fatty acids GC analysis as reported in [17]. The values were given as median (min; max). is the number of subjects. FAME (fatty acid methyl ester) was determined performing membrane phospholipid extraction, derivatization, and GC analysis as described. The identification of the peaks was performed using authentic samples as described [17, 18]. The optimal interval values are reported from [17], as found in a survey of healthy controls reported in the scientific literature and compared with a group of 2500 analyses obtained from the Italian population]. 9-trans-18:1 is considered. As described in [19], values less than 0.05 were considered statistically significant.
