Nutrition and Reproductive Health: Sperm versus Erythrocyte Lipidomic Profile and ω-3 Intake
Table 4
Fatty acid profile in sperm cells isolated from the human.
FAME
Samples before supplementation (; % rel)
Samples after supplementation (; % rel)
value
14:0
1.4 (0.83; 1.84)
1.41 (0.76; 2.55)
0.61
16:0
33.3 (30.23; 40.78)
33.79 (24.12; 35.41)
0.25
17:0
3.21 (1.28; 3.99)
2.08 (1.25; 2.82)
0.12
18:0
12.67 (7.55; 16.47)
10.33 (8.73; 27.07)
0.75
20:0
2.5 (1.02; 3.05)
1.54 (1.11; 2.63)
0.48
22:0
7.67 (2.05; 10.12)
4.53 (1.78; 12.54)
0.76
TOT. SFA
58.03 (52.06; 64.22)
55.01 (38.72; 66.74)
0.19
6c 16:1
1.38 (1.0; 1.78)
1.9 (0.65; 3.98)
>0.99
9c 16:1
3.99 (2.63; 6.31)
3.68 (1.72; 18.15)
>0.99
9c 18:1
9.85 (6.56; 12.85)
8.52 (7.2; 15.15)
0.44
11c 18:1
6.96 (3.77; 9.72)
4.79 (2.05; 19.27)
0.87
20:1
3.33 (0.57; 6.79)
3.83 (1.15; 11.87)
0.29
22:1
1.25 (0.33; 2.14)
0.88 (0.26; 1.56)
0.12
TOT. MUFA
27.48 (18.96; 30.67)
26.5 (20.29; 52.94)
0.54
SFA/MUFA
2.17 (1.84; 2.81)
2.17 (0.73; 3.01)
0.4
18:2
4.21 (3.32; 5.56)
5.0 (2.5; 6.19)
0.37
20:2
0.66 (0.16; 1.48)
0.36 (0.26; 1.25)
0.48
20:3
1.2 (0.37; 2.44)
1.21 (0.65; 2.54)
0.91
20:4 (arachidonic acid)
1.54 (0.91; 2.47)
1.23 (0.63; 3.21)
0.41
TOT. ω6
7.54 (6.77; 9.71)
8.12 (5.0; 10.27)
0.71
20:5 (EPA)
0.46 (0.16; 1.2)
0.31 (0.17; 0.59)
0.34
22:5
0.95 (0.44; 2.41)
0.62 (0.35; 3.72)
0.15
22:6 (DHA)
3.02 (1.46; 19.47)
3.5 (1.41; 10.4)
0.71
TOT. ω3
4.59 (3.02; 22.06)
4.45 (1.93; 11.79)
0.78
ω6/ω3
1.75 (0.34; 2.57)
1.59 (0.62; 3.3)
0.46
Trans fatty acid (9t-18:1)
0.18 (0; 0.65)
0.12 (0; 0.26)
0.24
The values of the fatty acids are reported as relative percentage (% rel) of the fatty acid peak areas detected in the GC analysis (>98% recognized peaks). The values were given as median (min; max). is the number of samples. FAME (fatty acid methyl ester) was determined performing membrane lipid extraction, derivatization, and GC analysis as described [17, 18]. values less than 0.05 were considered statistically significant.