Research Article

Nutrition and Reproductive Health: Sperm versus Erythrocyte Lipidomic Profile and ω-3 Intake

Table 4

Fatty acid profile in sperm cells isolated from the human.

FAMESamples before supplementation
(; % rel)
Samples after supplementation
(; % rel)
value

14:01.4 (0.83; 1.84)1.41 (0.76; 2.55)0.61
16:033.3 (30.23; 40.78)33.79 (24.12; 35.41)0.25
17:03.21 (1.28; 3.99)2.08 (1.25; 2.82)0.12
18:012.67 (7.55; 16.47)10.33 (8.73; 27.07)0.75
20:02.5 (1.02; 3.05)1.54 (1.11; 2.63)0.48
22:07.67 (2.05; 10.12)4.53 (1.78; 12.54)0.76
TOT. SFA58.03 (52.06; 64.22)55.01 (38.72; 66.74)0.19
6c 16:11.38 (1.0; 1.78)1.9 (0.65; 3.98)>0.99
9c 16:13.99 (2.63; 6.31)3.68 (1.72; 18.15)>0.99
9c 18:19.85 (6.56; 12.85)8.52 (7.2; 15.15)0.44
11c 18:16.96 (3.77; 9.72)4.79 (2.05; 19.27)0.87
20:13.33 (0.57; 6.79)3.83 (1.15; 11.87)0.29
22:11.25 (0.33; 2.14)0.88 (0.26; 1.56)0.12
TOT. MUFA27.48 (18.96; 30.67)26.5 (20.29; 52.94)0.54
SFA/MUFA2.17 (1.84; 2.81)2.17 (0.73; 3.01)0.4
18:24.21 (3.32; 5.56)5.0 (2.5; 6.19)0.37
20:20.66 (0.16; 1.48)0.36 (0.26; 1.25)0.48
20:31.2 (0.37; 2.44)1.21 (0.65; 2.54)0.91
20:4 (arachidonic acid)1.54 (0.91; 2.47)1.23 (0.63; 3.21)0.41
TOT. ω67.54 (6.77; 9.71)8.12 (5.0; 10.27)0.71
20:5 (EPA)0.46 (0.16; 1.2)0.31 (0.17; 0.59)0.34
22:50.95 (0.44; 2.41)0.62 (0.35; 3.72)0.15
22:6 (DHA)3.02 (1.46; 19.47)3.5 (1.41; 10.4)0.71
TOT. ω3 4.59 (3.02; 22.06)4.45 (1.93; 11.79)0.78
ω6/ω31.75 (0.34; 2.57)1.59 (0.62; 3.3)0.46
Trans fatty acid (9t-18:1)0.18 (0; 0.65)0.12 (0; 0.26)0.24

The values of the fatty acids are reported as relative percentage (% rel) of the fatty acid peak areas detected in the GC analysis (>98% recognized peaks). The values were given as median (min; max). is the number of samples. FAME (fatty acid methyl ester) was determined performing membrane lipid extraction, derivatization, and GC analysis as described [17, 18]. values less than 0.05 were considered statistically significant.