Uptake of Single-Walled Carbon Nanotubes Conjugated with DNA by Microvascular Endothelial Cells

Figure 1

A series of z-stack images demonstrating differential distribution of SWCNT-DNA-FITC (2 μg/mL) versus DNA-FITC (2 μg/mL) conjugates in microvascular ECs: (A) (a to i), representative optical section series of SWCNT-DNA-FITC conjugates in microvascular ECs at 24 hrs post incubation; (B) (j to r), representative optical section series of DNA-FITC present in microvascular ECs at 24 hrs post incubation; (C), sample 3-D image from a video (supplemental materials) acquired from ECs by a spinning confocal imaging system 48 hrs after incubation with SWCNT-DNA-FITC (2 μg/mL). The scale bar in (B) (o) is applicable to all images (a–r).