Control of Differentiation of Human Mesenchymal Stem Cells by Altering the Geometry of Nanofibers

Figure 3

Gene expression of the differentiated MSCs at day 3. Lane 1: MSC cultured in DMEM; lanes 2–4: MSCs cultured in OIM; lanes 5–7: MSC cultured in AIM. Cells were cultured in a dish (lanes 1, 2, and 5), aligned nanofibers (lanes 3 and 6), random nanofibers. (lanes 4 and 7) ALPL: alkaline phosphatase; RUNX2: runt-related transcription factor 2; SPP1: secreted phosphoprotein 1 (osteopontin); BGLAP: bone gamma-carboxyglutamate (gla) protein; COL1A1: collagen, type I, alpha 1; COL3A1: collagen, type III, alpha 1; FABP4: fatty acid binding protein 4; PPARG: peroxisome proliferator-activated receptor gamma; CEBPA: CEBPA CCAAT/enhancer binding protein (C/EBP), alpha; ADIPOQ: adiponectin, C1Q, and collagen domain containing; LPL: lipoprotein lipase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase (as standard).