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Figure 4: Differentiation of MSC on nanofiber in the mixed medium for 7 days. (A) Phase contrast images of differentiated MSC. MSCs were stained by alkaline phosphatase ((a)–(c)) and with Oil-Red-O. ((d)–(f)) Cells were cultured on a flat dish, ((a) and (d)) aligned fibers, ((b) and (e)) and random fibers. ((c) and (f)) Bar = 500 μm. (B) Quantitative analysis of osteoinduction. (C) Quantitative analysis of adipoinduction. (*: 𝑃 < 0 . 0 5 ; §: 𝑃 < 0 . 1 ).