Research Article

Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates

Figure 3

Determination of optimal coating concentration for pyrene-hydrazide labeled E. coli O157:H7 polyclonal antibody. CNT-FET circuits were coated with labeled antibody as described in Section 2 followed by addition of 10 μL of 107 cfu/mL E. coli O157:H7 in diH2O. Impedance was measured for 4 minutes after addition of the bacteria and plotted as the normalized impedance compared to baseline value of the individual circuit. Each point represents at least 5 measurements with standard error of the mean.
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