Stat3 Expression and Its Correlation with Proliferation and Apoptosis/Autophagy in Gliomas

Table 1

Scoring system for evaluation of immunostaining.

Marker		Category
	A	B	C
pStat3 and pAkt	Diffuse, <20%, + with or without foci	Diffuse, 20–50%, ++ with or without foci	Diffuse, >50% or >20%, +++ with multiple foci
Ki-67/MIB.1	<20%	20–30%	>30%
EGFR Amplification	Non-amplified	Non-amplified	More than 2 copies
EGFRwt	−	+/−	+
EGFRvIII	−	+/−	+
PTEN	Positive	—	Negative

Diffuse = homogeneous distribution of positive nuclei.
Foci = circumscribed small areas with a higher percentage of positive nuclei.
%  =  number of positive nuclei×100 nuclei.