Biochanin A Modulates Cell Viability, Invasion, and Growth Promoting Signaling Pathways in HER-2-Positive Breast Cancer Cells
Figure 7
Effect of biochanin A on MMP-2 and MMP-9 activity. (a) SK-BR-3 breast cancer cells were seeded in a T-75 flask in RPMI-1640 cell culture media (10% FBS) for 24 hours. Subsequently they were treated for 72 hours with biochanin A (20 and 50 M) in serum free conditions and MMP-2 and MMP-9 gelatinase enzyme activity was evaluated. The data indicate reduced MMP-9 activity with 50 M biochanin A (; ). C: control or no treatment; DMSO: vehicle; PL: protein ladder (Statistical analysis: One-way Anova, , *). (b) Representative gelatin gel for MMP-2 and MMP-9 activity.
