Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal

<table><i>Anti-Ln-332 antibody prevents A431 colony from dispersing by LPA treatment.</i> Cell colony dispersal assays were performed similarly to our previous studies (serum-starved A431 cells for 24 h, treated with 2 <svg height="9.6750002" id="M138" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
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<tspan style="font-size: 12.50px; " x="0" y="0">𝜇</tspan>
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</svg>M LPA), with the addition of an anti-Ln-332 antibody (BM165).  (a, b) We found that anti-Ln-332 antibody significantly blocked dispersal of LPA-treated colonies, compared to those colonies treated with LPA alone (<i>N</i> = 3; <i>P</i> &lt;  .05). LPA-treated A431 colony dispersal percentages in the absence (LPA) or presence of BM165 antibody (LPA + anti-Ln-332) are indicated as light-grey triangles or cross, respectively.  These results strongly support a general role for Ln-332 in regulating the “LPA effect”.  <svg height="11.525" id="M139" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 6.4749999 11.525" width="6.4749999" xmlns="http://www.w3.org/2000/svg">
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</svg><i>P</i> &lt;  .05, Two-way ANOVA (Bonferroni post test).</table>

Journal of Oncology

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Figure 6

Figure 6: Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal 