Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Gene</td><td align="center">Primer set</td><td align="center">Gene accession number</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left" rowspan="2">LAMA3</td><td align="center"><svg height="13.175" id="M42" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-GGCTCACTCTGTATTGTTGG</td><td align="center" rowspan="2">NM_000227</td></tr><tr><td align="center"><svg height="13.175" id="M43" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-ACAGAGACTGCTTTGGTGTG</td></tr><tr><td align="left" rowspan="2">LAMB3</td><td align="center"><svg height="13.175" id="M44" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-TGATGGACAGGATGAAAGAC</td><td align="center" rowspan="2">NM_000228</td></tr><tr><td align="center"><svg height="13.175" id="M45" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-GGAAGCTGTAGCATCACTTG</td></tr><tr><td align="left" rowspan="2">LAMC2</td><td align="center"><svg height="13.175" id="M46" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-GAAGCCCAGAAGGTTGATAC</td><td align="center" rowspan="2">NM_005562</td></tr><tr><td align="center"><svg height="13.175" id="M47" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-GTGAGTGTTCTGGAGCAAAG</td></tr><tr><td align="left" rowspan="2">GAPDH</td><td align="center"><svg height="13.175" id="M48" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-ATGACATCAAGAAGGTGGTG</td><td align="center" rowspan="2">NM_002046</td></tr><tr><td align="center"><svg height="13.175" id="M49" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-CTGTAGCCAAATTCGTTGTC</td></tr><tr><td align="left" rowspan="2">PPIA</td><td align="center"><svg height="13.175" id="M50" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-CAAATGCTGGACCCAACACA</td><td align="center" rowspan="2">NM_021130</td></tr><tr><td align="center"><svg height="13.175" id="M51" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
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</svg>-TGCCATCCAACCACTCAGTC</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<i>Primers used for quantitative RT-PCR. </i>For RT-PCR, we analyzed samples on an MiQ machine using a FastStart SYBR Green Master Mix, with the following primer sets.

Journal of Oncology

tab1

Table 1

Table 1: Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal 