The Synergistic Effect of Conditional Pten Loss and Oncogenic K-ras Mutation on Endometrial Cancer Development Occurs via Decreased Progesterone Receptor Action

Figure 1

Analysis of conditionally ablated Pten and oncogenic K-ras mutation in the murine uterus. (a) Western blot analysis of PTEN in whole uterine extracts from Pten^f/f and Pten^d/d mice. (b) Immunohistochemical analysis for PTEN in Pten^f/f and Pten^d/dmice uteri. Four-week-old Pten^f/f and Pten^d/d mice were used for Western blot analysis and immunohistochemical analysis. These experiments demonstrate that Pten is conditionally ablated in the uteri of these mice. Scale bar: 50 𝜇m. (c) PR-cre mediated recombination of K-ras^G12D in uterus. The mutant K-ras G12D allele contains a HindIII restriction site engineered in exon 1, which is absent in the wild-type allele. Therefore, digestion of the 488-bp products generates 300-bp and 148-bp restriction fragments in the mutant but not in the wild-type PCR product.