232831.fig.001a
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232831.fig.001b
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Figure 1: 3D cocultures of ARCaPE or ARCaPM with HS-27a cells show E-cadherin expression. (a) 1 × 1 0 7 ARCaPE or ARCaPM were cocultured with HS-27a cells in RWV for 3 days. Immunohistochemistry of organoids was stained with anti-E-cadherin or N-cadherin antibody. (b) 2D Cocultures of HS-27a were preformed utilizing a total of 50,000 cm2/HS-27a fibroblasts, after which 20,000 cm2 ARCaPE or ARCaPM were seeded on top of the fibroblast monolayer. The cocultures were maintained in serum-free medium for 1 or 4 days. Immunocytochemistry of cocultures over these time periods was performed utilizing anti-E-cadherin and N-cadherin antibodies. Shown are the EMT/MET of ARCaPEcells (top panels) and MErT of ARCaPMcells (bottom panels).