232831.fig.002a
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232831.fig.002b
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232831.fig.002c
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232831.fig.002d
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Figure 2: ARCaPE cells show a growth and colony forming capacity advantage in presence of HS-27a cells. (a) and (b) ARCaPM cells were cocultured in the presence of GFP-HS-27a cells over a 6-day period. Growth of RFP. ARCaPE or ARCaPM human prostate cancer cells was assessed by RFUs (relative fluorescent units) in the presence cocultures over a 6-day period. Results are means ± SE of three independent experiments. 𝑃 < . 0 5 (students 𝑡 -test) compared to cell number at day 1 ± SEM. (c) Clonogenic colony forming capacity of ARCaPE and ARCaPM prostate cancer cell after coculture ± SEM. ARCaPM data were normalized to ARCaPM control, and ARCaPE data were normalized to ARCaPE control (Note HS-27a induced slightly (1.35x) the growth of ARCaPM cells but markedly (8x) the growth of ARCaPE cells.). (d) ARCaPE or ARCaPM cells were cocultured with HS-27a cells. Shown are phase contrast images of colonies formed in the clonogenic assay.