Tumor-Stromal Interactions Influence Radiation Sensitivity in Epithelial- versus Mesenchymal-Like Prostate Cancer Cells
Figure 3
Cocultured ARCaPE cells gain cell colony forming capacity and radiation resistance when grown with bone and prostate stromal cells. (a) ARCaPE or ARCaPM cocultured cells were irradiated 24 hours after coculture with HS-27a cells and cancer cell colony forming capacity was assayed using clonogenic assay. Results are means ± SE of three independent experiments. ARCaPM experimental data are normalized to ARCaPM control and ARCaPE experimental data are normalized to ARCaPE control (a). ARCaPE cells cocultured with prostate stromal fibroblasts Pt-C (Cancer associated fibroblasts) or Pt-N (Normal/benign fibroblasts) were irradiated and compared to nonirradiated cocultures. Cell colony forming capacity was assayed by clonogenic assay. Data are normalized to ARCaPE control levels. (b) ARCaPM cells cocultured with Pt-C or Pt-N were irradiated and compared to nonirradiated cocultures (c). Cell colony forming capacity was assayed by clonogenic assay. Data are normalized to ARCaPM control levels.
