An Active Form of Sphingosine Kinase-1 Is Released in the Extracellular Medium as Component of Membrane Vesicles Shed by Two Human Tumor Cell Lines
Figure 1
Comparative analysis of Integrin, SphK-2, and SphK-1 immunolocalization. (a) Localization in 8701-BC cells. (b) Localization in Sk-Hep1 cells. Line a: Immunolocalization of Integrin and SphK-2 showing a different distribution of the two molecules. Line b: Immunolocalization of Integrin and SphK-1. Arrows indicate colocalization areas. Integrin was detected using FITC-conjugated secondary antibodies and SphK-2 and SphK-1 using Texas red-conjugated secondary antibodies. Arrows indicate colocalization areas.
