Cleavage of E-Cadherin by Matrix Metalloproteinase-7 Promotes Cellular Proliferation in Nontransformed Cell Lines via Activation of RhoA
Figure 2
Epithelial polarization is negatively impacted by MMP-7. (a) Analysis of MDCK polarization in response to the addition of basolateral or apical exogenous MMP-7 (100 ng/mL) to the lower or upper compartment of the transwell chamber, as determined by ANOVA followed by Bonferroni’s post hoc test. Dashed line represents the resistance level at which MDCKs are polarized [7]. (b) Analysis of C57MG cell line transepithelial resistance in response to the expression of MMP-7. Confluent (day 8) MMP-7 expressing clones (M14 and M37) growing on a transwell membrane never reached a resistance that was comparable to that of the empty vector control C57MG clones.
