Cleavage of E-Cadherin by Matrix Metalloproteinase-7 Promotes Cellular Proliferation in Nontransformed Cell Lines via Activation of RhoA
Figure 3
MMP-7 enhances the proliferation of nontransformed epithelial cells. (a) Analysis of proliferation using [3H]-thymidine of confluent MDCK (grown on 0.4 m transwell membrane) upon addition of exogenous MMP-7 (100 ng/mL) to the basolateral (lower chamber) or apical surface (upper chamber). as determined by one-way ANOVA with Bonferroni post hoc test. The values represent scintillation counts corrected for amount of protein present. (b) Representative photomicrographs of vector control (L2/L4) and MMP-7 expressing (M14/37) C57MG cells under subconfluent and confluent conditions (). (c) The average cell counts of vector control (L2 and L4) and MMP-7 (M14 and M37) expressing C57MG cell lines over time. ANOVA analysis followed by Bonferroni’s post hoc analysis of the numbers of cells in each group at the day 10 time point revealed that the M14 and M37 cell lines reached significantly higher numbers of cells ().
