Cleavage of E-Cadherin by Matrix Metalloproteinase-7 Promotes Cellular Proliferation in Nontransformed Cell Lines via Activation of RhoA
Figure 4
MMP-7 promotes E-cadherin processing. (a) Detection of the ectodomain of E-cadherin (80 kDa, open arrow head) in the conditioned media of MDCK cells in the presence () or absence () of exogenous MMP-7 (100 ng/mL). (b) Analysis of E-cadherin processing in the empty vector control (L2 and L4) and MMP-7 (M14 and M37) expressing cell lines. Open arrow head indicates the ectodomain of E-cadherin (80 kDa) in conditioned media, while arrow and arrow head indicate full length E-cadherin (120 kDa) and intracellular fragment of E-cadherin (40 kDa), respectively, in C57MG cell line lysates.
