Cleavage of E-Cadherin by Matrix Metalloproteinase-7 Promotes Cellular Proliferation in Nontransformed Cell Lines via Activation of RhoA
Figure 5
Epithelial proliferation is mediated by MMP-7 processing of E-cadherin. (a) Growth of the C57MG cell lines in the presence or absence of the broad spectrum metalloproteinase inhibitor (BB-94) over a 24-hour period was determined by MTT assay. Asterisk denotes that the proliferation of the MMP-7 expressing cell lines (M14/M37) is significantly higher than the empty vector controls (L2/L4), while double asterisks denote that proliferation is significantly inhibited in the presence of BB-94 compared to the control conditions, . Significance was determined using ANOVA followed by Tukey’s post hoc test. (b) The effect of E-cadherin on cell growth in the absence (IgG) or presence of an E-cadherin blocking antibody (-E-cad) was measured using an MTT assay. Asterisk denotes significantly higher growth in the presence of the E-cadherin blocking antibody in comparison to the IgG treated controls, . Significance was determined using ANOVA followed by Tukey’s post hoc test.
