Loss of miR-200c: A Marker of Aggressiveness and Chemoresistance in Female Reproductive Cancers

Figure 1

Reciprocal repression between ZEB1 and miR-200c occurs in some, but not all cell types. (a) Schematic of the mutual repression of ZEB1 and miR-200c. (b) Western blotting for ZEB1 and $\alpha$-tubulin loading control, and real time RT-PCR for miR-200c in Hey ovarian cells transiently transfected with a miR-200c mimic. Real time RT-PCR for miR-200c and western blotting for ZEB1, E-cadherin, and α-tubulin in MDA-MB-231 breast cancer cells (c), and Hec50 endometrial cancer cells (d) stably transfected with shRNA lentiviral vector targeting ZEB1 (shZEB), luciferase (shLuciferase), or the empty vector (shRNA EV).

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