Loss of miR-200c: A Marker of Aggressiveness and Chemoresistance in Female Reproductive Cancers

Figure 5

Restoration of TUBB3 reverses miR-200c-mediated enhanced chemosensitivity to paclitaxel. (a) Western blots for ZEB1, E-cadherin, TUBB3, and α-tubulin in Hec50 cells transiently transfected with a miR-200c mimic or negative controls and an expression vector for TUBB3 or empty vector. (b) Real time RT-PCR for miR-200c. Cell death ELISA for cells transfected with a miR-200c mimic and an empty vector (c) or TUBB3 expression vector (d) treated with various concentrations of paclitaxel. Asterisks indicate statistically significant difference (P $<$ .05, Student’s $t$-test) versus negative controls.

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