Inhibition of Melanoma Angiogenesis by Telomere Homolog Oligonucleotides
Figure 1
T-oligo treatment down-regulates expression of VEGF, Ang-1, Ang-2 and decreases VEGF production in MM-AN cells. MM-AN cells were treated once at time 0 with either 40 M T-oligo or diluent alone. (a) Western blot analysis of VEGF protein level. (b) Densitometric analysis of VEGF protein levels (after loading adjustment against actin expression) represented as a percent of time 0 level. Graphs represent pooled data (mean ± SEM) from three independent experiments. (c) and (d) Quantitative real time-PCR (qRT-PCR) of MM-AN cells treated with either 40 M T-oligo or diluent alone. Results are presented as percent of time 0 (set at 100%) and examined over 48 hours for both control and T-oligo-treated cells. ANG-1 gene expression. (c) ANG-2 gene expression. (d) These experiments are repeated twice with similar results. (e) MM-AN cells were treated with 40 M T-oligo or diluent alone. The culture medium was collected after 24, 48, and 72 hours. Cumulative VEGF protein released into the medium was measured by ELISA. Results represent data pooled from triplicate dishes for each time point and treatment condition. Changes over time are calculated as percent of the 24 hours values (set at 100%).
