Hyperphosphorylated FAK Delocalizes from Focal Adhesions to Membrane Ruffles

Figure 3

Analysis of FA behavior upon FAK hyperphosphorylation. (a) Confocal time-lapse images of FAK/Ycam expressed cells were collected every 5 minutes for 1 hour after PV addition. Confocal images of living cells at 0, 20, 40, and 60 minutes are shown. Arrowheads, labeled 1 to 4, indicate areas of FA disassembly. Scale bar, 10 $\mu$m. (b) Image J software was used to apply a filter and a size constraint to FAs and the image shown results from the merging of images at $t=60$ (black) and $t=0$ (white). Newly formed FA (black) and dissociated FA (white). Graph represents the number of FA/cell that was measured by the segmentation/size criteria process both before PV ($t=0$, white bar) and after 60 minutes PV treatment ($t=60$, black bar) from 8 cells (mean $\pm$ s.e.m.). Note the significant decrease in the number of FAs in cells treated with PV compared to untreated cells ($P<.05$).

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(b)