Hyperphosphorylated FAK Delocalizes from Focal Adhesions to Membrane Ruffles
Figure 5
Increased phosphorylated FAK at cell membrane. (a) Images of PV-treated cells were taken by confocal microscopy at 5 minutes intervals for 30 minutes, then cells were fixed and immunolabeled for p-Tyr397-FAK (Rhodamine Red-X). Scale bar, 20 m. Note membrane ruffle formation (arrowheads) and loss of FAs (arrows) upon addition of PV. Moreover, membrane ruffles show strong P-Tyr397-FAK labeling (right panel, red). (b) Boxed regions in panel (a) are magnified in panel (b) and correspond to FAK/Ycam (green) and p-Tyr397-FAK (red) signals at 30 minutes after PV. Fluorescence intensity analyses were quantified at the membrane region. Fluorescence intensity was measured along the 10 m line (red) using Plot Profile, Image J software, with grey values ranging from 0 to 256. Note the increase in phospho-FAK signal at the cell membrane compared to total FAK (maximal intensities indicated by black arrowheads).
