CXCL7-Mediated Stimulation of Lymphangiogenic Factors VEGF-C, VEGF-D in Human Breast Cancer Cells
Figure 2
SB225005 blocked VEGF-C and VEG-D mRNA expression by CXCL7-transfected MCF10AT cells. CXCL7-stably transfected MCF10AT cells were cultured with and without SB225002 (1.1 M). After 48-hour treatment, the media and the cells were collected. (a) Quantitative analysis of VEGF-C and VEGF-D mRNA expression. Total RNA was extracted, and real-time qRT-PCR was performed. -actin was applied as internal control. Triplicate determinations were performed. The differences between the cell lines were significant (). (b) Quantitative analysis of VEGF-C and VEGF-D protein secretion by the CXCL7-stable transfected MCF10AT cells. Protein concentration in CM was measured by ELISA using human VEGF-C and VEGF-D ELISA Development System. Measurements were done at least in duplicate for 2 dilutions. The optical density of each well was determined using plate reader by subtracting the reading at 570 nm from the reading at 450 nm. VEGF-C/D content in CM of SB225002-treated group was significantly lower than untreated group ().
