CXCL7-Mediated Stimulation of Lymphangiogenic Factors VEGF-C, VEGF-D in Human Breast Cancer Cells
Figure 5
ERK1/2 mitogen-activated protein kinase is not active in CXCL7 stable transfected MCF10AT cells. Briefly, the cells were harvested in PBS, counted and lysed in the RIPA buffer with protease inhibitor cocktail. Protein concentration was determined for all samples using the Bio-Rad protein assay. The equal-volume samples (50 g) were separated by SDS-PAGE on a 10% polyacrylamide gel and transferred onto nitrocellulose membrane. Immunodetection was performed using pERK1/2 and ERK1/2, then developed by ECL. Stable CXCL7 transfected MCF10AT cells did not induce ERK1/2 phosphorylation compared to the vector transfected control cells. Furthermore, the expression of ERK1/2 was the same in stable CXCL7 transfected MCF10AT cells compared to vector controls.
