Ganglioside GM3 Is Antiangiogenic in Malignant Brain Cancer

Figure 6

GM3 reduces CT-2A tumor vascularity when added to the tumor microenvironment. Small fragments of the CT-2A tumor were grown in Matrigel that contained either no GM3 (control) or GM3 ($40\hspace{0.17em}\mu \text{M}$). The tumor was grown in Matrigel for approximately two weeks in the flank of the syngeneic host C57BL/6 mice according to our standard procedures [7, 34]. Florid vascularization and the number and size thrombotic vessels were noticeably less in the presence than in the absence of GM3. Similar results were found in two independent experiments.