Breast Carcinoma Cells in Primary Tumors and Effusions Have Different Gene Array Profiles
Figure 3
Validation by PCR and Western blotting. (a): DCN mRNA expression in effusions and solid primary tumors, showing higher expression in the latter specimen type (). DCN levels were measured by qRT-PCR using specific primers for decorin A1, A2 and B isoformes. The pool sample consisting of cDNA from five primary tumors and five effusion samples served as a positive control. DCN mRNA expression levels were normalized to RPLPO reference gene expression levels. (b): BCAR1 mRNA relative expression levels in primary tumors and effusions. Gene expression level was measured by semiquantitative RT-PCR and normalized to relative 28s expression. The MCF-7 breast carcinoma cell line served as positive control. BCAR1 levels were significantly higher in effusions (). (c): VIL2 expression in effusions in comparison to primary carcinomas. VIL2 mRNA levels were measured by RT-PCR with specific primers and normalized to relative 28s expression. Human HT1080 fibrosarcoma cell line served as a positive control. VIL2 levels were significantly higher in effusions (). (d): Total Ezrin protein expression in effusions in comparison to primary carcinomas. Ezrin protein levels were analyzed using specific antibodies and normalized to total beta-actin levels. Ezrin levels were significantly higher in effusions ().
