Short-Term Exposure to Tobacco Toxins Alters Expression of
Multiple Proliferation Gene Markers in Primary Human Bronchial
Epithelial Cell Cultures
Table 1
Tobacco carcinogens and dilutions used.
No.
Tobacco carcinogens
Toxin range: ng/cigarette
Working concentrations
Solvent used for stock soln.
High ng/ml
Med#ng/ml
Lowng/ml
(1)
Nickel Sulphate**
0–510
800
500
200
Water
(2)
Cadmium Chloride
0–6670
1200
700
200
Water
(3)
Chromium Chloride
0.2–500
800
500
200
Water
(4)
Sodium Selenite
0–1400
1900
1400
900
Water
(5)
Benzo(b)fluoranthene**
4–22
44
22
2
Acetone
(6)
Indeno(1,2,3,-d)pyrene
4–20
40
20
2
ETOH
(7)
Ethyl Carbamate
20–38
80
40
2
ETOH
(8)
Dibenz(a,h)anthracene*
4
12
4
1
Toluene
(9)
N-Nitrosodiethylamine**
0–2.8
9
3
1
ETOH
(10)
5-Methylchrysene
0.6
3
1
0.25
ETOH
(11)
Dibenzopyrene
1.7–3.2
9
3
1
ETOH
(12)
Dibenz(a,h)acridine
0.1
3
0.1
0.25
Acetone
(13)
NNK**
80–770
1300
800
300
Water
(14)
Benzo(k)fluoranthene*
6–12
24
12
2
Toluene
*Excluded from morphometry and immunohistochemical analysis due to cytotoxicity.
**Used in immunohistochemistry only. #Equivalent volume of solvent/mL BEGM used as solvent (S) control in NSE staining only.
