Research Article

Antimyeloma Effects of the Heat Shock Protein 70 Molecular Chaperone Inhibitor MAL3-101

Figure 2

MAL3-101 induces cell cycle arrest and caspase-mediated apoptosis in the multiple myeloma (MM) cell line NCI-H929. (a) MM cell lines were exposed to 10 μM of MAL3-101 for 40 h, and the fold change in apoptosis in MAL3-101-treated versus control, DMSO-treated cells was determined by flow cytometry. Data are presented as means and SDs from three independent experiments; * . (b) The percentages of NCI-H929 cells in the and phases of the cell cycle are indicated in each panel. This result is representative of three independent experiments that yielded similar results. (c) NCI-H929 cells were exposed to 10 μM MAL3-101 for the indicated culture periods and immunoblotted with primary antibodies to detect caspase-3 and poly(ADP-ribose) polymerase (PARP). To ensure equal loading, β-actin levels were examined as a control. Bands corresponding to intact and cleaved caspase-3 and PARP are indicated. The data are representative of four experiments.
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