Antimyeloma Effects of the Heat Shock Protein 70 Molecular Chaperone Inhibitor MAL3-101
Figure 5
The unfolded protein response is not induced in NCI-H929 cells in response to chaperone and/or proteasome inhibition. RT-PCR analysis was performed to assess XBP-1 mRNA splicing in NCI-H929 cells, and arrows on the right indicate the 285 bp unspliced (XPB-1u) and the 259 bp spliced (XPB-1s) mRNA. (a) NCI-H929 cells were exposed for 4 h to MAL3-101, MG-132, 17AAG, or the indicated combination of the compounds, and total RNA was extracted for RT-PCR amplification. NCI-H929 cells treated for 4 h with 5 μg/mL tunicamycin (TM) as a positive control induced XBP-1 mRNA splicing. (b) NCI-H929 cells were exposed for 0, 24, 48, and 72 h to 30 μM MAL3-101, and total mRNA was extracted for RT-PCR amplification.
