Antimyeloma Effects of the Heat Shock Protein 70 Molecular Chaperone Inhibitor MAL3-101

Table 1

MM cell line NCI-H929 is a high secretor of monoclonal IG. Multiple myeloma (MM) cell lines were grown at 1×10⁶ cells/mL for 16 h, and 500 ng total protein from cell lysates and 500 ng from serum-free supernatants were used in ELISAs to determine intracellular and secreted immunoglobulin (IG) light-chain (LC) levels, respectively. Relative LC secretion is shown as the proportion of secreted versus intracellular LC levels. Data are presented as the mean ± SD from 3 independent experiments.

MM cell line	Light chain (LC) produced	Secreted LC (ng/10^6 cells)	Intracellular LC (ng/10^6 cells)	Relative LC secretion (secreted/intracellular)
NCI-H929	κ	505 ± 22	93 ± 9.9	5.46 ± 0.4
U266	λ	639 ± 30	387 ± 32	1.66 ± 0.2
RPMI-8226	λ	56 ± 51	1271 ± 18	0.16 ± 0.1