Molecular Mechanisms of Cigarette Smoke-Induced Proliferation of Lung Cells and Prevention by Vitamin C

Figure 8

AECS/p-BQ exposure results in aberrant phosphorylation of the EGFR. Serum-starved A549 cells were either nontreated (NT) or exposed to 100 ng/mL EGF for 5 min, 2 μL/mL AECS or 200 ng/mL p-BQ for 1 hr. Cells were lysed, and the EGFR was immunoprecipitated (IP) from the cell lysates using anti-EGFR antibody. Immunoprecipitated proteins were separated by SDS-PAGE, transferred to PVDF membrane, and immunoblotted (IB) with indicated EGFR phosphotyrosine-specific antibodies or anti-EGFR antibodies.