Bmi-1 Regulates Snail Expression and Promotes Metastasis Ability in Head and Neck Squamous Cancer-Derived ALDH1 Positive Cells
Figure 3
Stemness properties were enhanced in HNSCC-ALDH1- cells when Bmi-1 was overexpressed. (a) Gene expression microarray analysis (Gene tree) for altered genes differentially expressed in Bmi-1-overexpressing HNSCC-ALDH1- cells compared to HNSCC-ALDH1- cells by a hierarchy heat map. The time dependent changes of altered genes are presented on a log scale of expression values provided by GeneSpring GX software. (b) Principle component analysis (PCA) demonstrated that overexpression of Bmi-1 in HNSCC-ALDH1- cells could enhance the gene signature of embryonic stem cells (ESCs) in HNSCC-ALDH1- cells. (c) Multidimensional scaling analysis. Average lineage transcriptome distances between HNSCC-ALDH1+, HNSCC-ALDH1-, HNSCC-ALDH1+/sh-Bmi-1, and HNSCC-ALDH1-/ cells. . (d) Transcripts of Oct-4, Nanog, Sox2, KLF4, Lin28, Snail, Slug, MDR-1, and ABCG2 in HNSCC-ALDH1- and HNSCC-ALDH1-/Bmi- cells (: ALDH1- versus Bmi-1-overexpressing ALDH1-).
