Research Article
Bmi-1 Regulates Snail Expression and Promotes Metastasis Ability in Head and Neck Squamous Cancer-Derived ALDH1 Positive Cells
Table 2
The sequences for the primers of quantitative RT-PCR.
| | Gene(Accession No.) | Primer Sequence ( to ) | Product size (bp) | Tm (°C) |
| | Oct-4(NM_002701) | F: GTGGAGAGCAACTCCGATG | 86 | 60 | | R: TGCTCCAGCTTCTCCTTCTC |
| | Nanog(NM_024865) | F: ATTCAGGACAGCCCTGATTCTTC | 76 | 60 | | R: TTTTTGCGACACTCTTCTCTGC |
| | SOX-2(NM_003106) | F: CGAGTGGAAACTTTTGTCGGA | 74 | 60 | | R: TGTGCAGCGCTCGCAG |
| | Musashi(NM_002442) | F: TCCCTCGGCGAGCACA | 64 | 60 | | R: GACAGCCCCCCCACAAA |
| | c-Myc(NM_002467) | F: GGAACGAGCTAAAACGGAGCT | 71 | 55 | | R: GGCCTTTTCATTGTTTTCCAACT |
| | β-catenin(NM_001904) | F: CCAGCCGACACCAAGAAG | 130 | 55 | | R: CGAATCAATCCAACAGTAGCC |
| | Bmi1(NM_ 005180) | F：AAATGCTGGAGAACTGGAAAG | 124 | 50 | | R：CTGTGGATGAGGAGACTGC |
| | Nestin(NM_006617) | F: AGGAGGAGTTGGGTTCTG | 112 | 50 | | R: GGAGTGGAGTCTGGAAGG |
| | Snail(NM_005985) | F:GCTGCCAATGCTCATCTGGGACTCT | 300 | 55 | | R: TTGAAGGGCTTTCGAGCCTGGAGAT |
| | Slug(NM_003068) | F: GTGATTATTTCCCCGTATCTCTAT | 292 | 50 | | R: CAATGGCATGGGGGTCTGAAAG |
| | MDR-1 (NM_000927) | F: TGGCAAAGAAATAAAGCGACTGA | 76 | 60 | | R: CAGGATGGGCTCCTGGG |
| | MRP-1(X60111) | F: GCTTCCTCTTGGTGATATTCG | 176 | 50 | | R: GCAGTTCAACGCATAGTGG |
| | ABCG2(NM_004827) | F: CATGTACTGGCGAAGAATATTTGGT | 74 | 60 | | R: CACGTGATTCTTCCACAAGCC |
| | GAPDH(NM_002046) | F: CATCATCCCTGCCTCTACTG | 180 | 60 | | R: GCCTGCTTCACCACCTTC |
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