Prevalence of Epithelial Ovarian Cancer Stem Cells Correlates with Recurrence in Early-Stage Ovarian Cancer

Figure 2

CD44+ EOC cells undergo in vivo differentiation into CD44− cells. Flow cytometry analysis of CD44+ cells stable transfected with a lentivirus expressing the fluorescent protein Tomato (red). (a) Cells prior to injection into the mice are 99.5% double positive for CD44 and Tomato. (b) 95.5% of the cells isolated from the tumor remain positive for the fluorescence protein Tomato but are negative to CD44. Only 4.5% of the injected cells remained double positive.