Gene Expression Differences between Enriched Normal and Chronic Myelogenous Leukemia Quiescent Stem/Progenitor Cells and Correlations with Biological Abnormalities
Figure 5
(a) CFU-GM and CFU-E/BFU-E colony formation by CD34+ G0 CD133/2 positive and negative cells, enriched from 3 pooled cord blood samples when stimulated by KL+FL+TPO and the same GFs + G-CSF+GM-CSF + IL-3 + IL-6. No EPO was added. C.E. = % total cloning efficiency. (b) Comparison of three pooled cord blood CD34+ G0 and G1/S/G2/M CD133/2 positive and CD133/2 negative cells ability to form CFU-GM and CFU-E/BFU-E when stimulated by the same 3 or 7 cytokines without EPO as in Figure 5(a). The total C.E.s of the enriched G0 and G1/S/G2/M cells from these pooled cord blood samples are lower than those in Figure 5(a), but again only the CD133/2 negative cells of both the G0 and G1/S/G2/M fractions formed erythroid colonies.
