Gene Expression Differences between Enriched Normal and Chronic Myelogenous Leukemia Quiescent Stem/Progenitor Cells and Correlations with Biological Abnormalities
Figure 7
(a) Expression of surface antigens determined by flow cytometry of normal mononuclear cells (MNCs) after Ficoll Hypaque separation from a normal frozen-thawed mobilized peripheral blood sample compared to expression of total CD34 cells after two enrichments of the same MNCs on Miltenyi columns as described in Methods. Insufficient numbers of CD34+ cells were recovered to permit comparison of additional antigens. (b) Changes in cell surface antigen expression in total CD34+ cells enriched from a sample of frozen thawed mononuclear cells from normal mobilized peripheral blood during 12 days of culture. The cells were stimulated to proliferate with the two combinations of cytokines shown in the concentrations stated under Methods.
