Survivin Selectively Modulates Genes Deregulated in Human Leukemia Stem Cells
Figure 1
The effect of Survivin deletion on the number of KSL cells transformed by ITD-Flt3 (a) The KSL cells transformed by ITD-Flt3 were FACS sorted using GFP as a marker. Marrow cells derived from littermate control Survivinflox/flox or CreER-Survivinflox/flox mice were transduced with ITD-Flt3 (N51) in MSCV-IRES-EGFP vector. Seventy-two hours after transduction, the medium was replaced with IMDM containing 10%FBS and the cells were incubated for 14 days in the presence of 1uM of 4OH-Tamoxifen without hematopoietic growth factors. GFP+, c-kit+, Sca-1+, and lineage-negative (KSL) cells shown in red box were sorted on day 14 by FACSAria (BD Biosciences). (b) The number of whole cells and KSL cells derived from Survivinflox/flox or CreER-Survivinflox/flox marrow cells transduced with ITD-Flt3 cultured with 4OH-tamoxifen in the absence of any growth factors was quantitiated in triplicate wells of each sample averaged from 2 independent experiments. * compared to control Survivinflox/flox.
