(a) Genes upregulated in LSC but downregulated in ITD-Flt3+ KSL cells deleted with Survivin (55 genes).Symbol Name Fold change Acaa2 acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) (Acaa2), [NM_177470]
−3.7
Ankrd12 Mus musculus 15 days embryo head cDNA, RIKEN full-length enriched library, clone:D930020E11 product: unknown EST, full insert sequence. [AK086311]
−5.5
Ankrd28 Mus musculus 2 days neonate thymus thymic cells cDNA, RIKEN full-length enriched library, clone:E430019N21 product: unknown EST, full insert sequence. [AK088541]
−2.9
Ap1g2 adaptor protein complex AP-1, gamma 2 subunit (Ap1g2), [NM_007455]
−5.2
Arhgef18 rho/rac guanine nucleotide exchange factor (GEF) 18 (Arhgef18), [NM_133962]
−3.6
Arrdc1 arrestin domain containing 1 (Arrdc1), [NM_178408]
−2.1
Atp11a ATPase, class VI, type 11A, [AK006628]
−2.6
BGLAP bone gamma carboxyglutamate protein 1 (Bglap1), [NM_007541]
−10.2
Cars cysteinyl-tRNA synthetase (Cars), [NM_013742]
−2.3
Ccr1 chemokine (C-C motif) receptor 1 (Ccr1), [NM_009912]
−3.5
Cd96 CD96 antigen (Cd96), [NM_032465]
−2.1
Cfp complement factor properdin (Cfp), [NM_008823]
−4.3
Cyyr1 cysteine and tyrosine-rich protein 1 (Cyyr1), [NM_144853]
−2.7
Dpagt1 dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (Dpagt1), [NM_007875]
−2.8
Dpp9 dipeptidylpeptidase 9 (Dpp9), [NM_172624]
−2.4
Elovl1 elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 1 (Elovl1), [NM_019422]
−2.5
Ets2 E26 avian leukemia oncogene 2, 3′ domain (Ets2), [NM_011809]
−2.5
Fgr Mus musculus Gardner-Rasheed feline sarcoma viral (Fgr) oncogene homolog (Fgr), mRNA [NM_010208]
−2.9
Gfi1 growth factor independent 1 (Gfi1), [NM_010278]
−2.7
Gga2 golgi associated, gamma adaptin ear containing, ARF binding protein 2 (Gga2), [NM_028758]
−2.5
Git2 G protein-coupled receptor kinase-interactor 2, [AK017943]
−3.1
Hdac10 histone deacetylase 10 (Hdac10), [NM_199198]
−4.1
Ipo13 importin 13 (Ipo13), [NM_146152]
−2.3
Isyna1 myo-inositol 1-phosphate synthase A1 (Isyna1), [NM_023627]
−2.5
Klhl17 kelch-like 17 (Drosophila) (Klhl17), [NM_198305]
−4.8
Lars2 leucyl-tRNA synthetase, mitochondrial (Lars2), nuclear gene encoding mitochondrial protein, [NM_153168]
−3.6
Lrrk1 leucine-rich repeat kinase 1 (Lrrk1), [NM_146191]
−2.7
Lztr1 leucine-zipper-like transcriptional regulator, 1 (Lztr1), [NM_025808]
−3.4
Mapk8ip3 mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3), [NM_013931]
−2.9
Mcm8 minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8), [NM_025676]
−2.4
Med25 TCBAP0758 protein (Fragment) homolog [Homo sapiens], [AK165760]
−5.4
Mr1 laminin receptor 1 (ribosomal protein SA) (Lamr1), [NM_011029]
−2.6
Napa N-ethylmaleimide sensitive fusion protein attachment protein alpha (Napa), [NM_025898]
−3.0
Ndrg1 N-myc downstream regulated gene 1 (Ndrg1), [NM_010884]
−3.3
Nsfl1c NSFL1 (p97) cofactor (p47) (Nsfl1c), [NM_198326]
−2.2
Otud5 bZ30I22.1 (Novel protein similar to human FLJ12550) (Fragment) homolog [Brachydanio rerio], [AK169921]
−3.1
Pank4 pantothenate kinase 4 (Pank4), [NM_172990]
−3.0
Pgls 6-phosphogluconolactonase, [AK158308]
−5.6
Phkg2 phosphorylase kinase, gamma 2 (testis) (Phkg2), [NM_026888]
−3.3
Pmaip1 phorbol-12-myristate-13-acetate-induced protein 1 (Pmaip1), [NM_021451]
−6.6
Pola2 polymerase (DNA directed), alpha 2 (Pola2), [NM_008893]
−2.7
Polg polymerase (DNA directed), gamma 2, accessory subunit (Polg2), [NM_015810]
−3.2
Rnf126 ring finger protein 126 (Rnf126), [NM_144528]
−2.1
Ropn1l ropporin 1-like (Ropn1l), [NM_145852]
−6.8
Rtel1 regulator of telomere elongation helicase 1 (Rtel1), [NM_001001882]
−4.3
Slc1a5 solute carrier family 1 (neutral amino acid transporter), member 5 (Slc1a5), [NM_009201]
−2.3
Slc25a28 solute carrier family 25, member 28 (Slc25a28), [NM_145156]
−2.9
Snapc4 small nuclear RNA activating complex, polypeptide 4 (Snapc4), [NM_172339]
−2.7
Solh small optic lobes homolog (Drosophila) (Solh), [NM_015830]
−3.9
Synj1 PREDICTED: synaptojanin 1 (Synj1), [XM_358889]
−2.8
Trim21 tripartite motif protein 21 (Trim21), [NM_009277]
−11.4
Trpm2 transient receptor potential cation channel, subfamily M, member 2 (Trpm2), [NM_138301]
−4.2
Trpm7 transient receptor potential cation channel, subfamily M, member 7 (Trpm7), [NM_021450]
−3.2
Ube3b ubiquitin protein ligase E3B (Ube3b), [NM_054093]
−2.8
Upp1 uridine phosphorylase 1 (Upp1), [NM_009477]
−3.4
Fold change: ITD-Flt3+ KLS cells derived from CreER-Survivinfx/fx compared to control Survivinfx/fx in two experiments. (b) Genes downregulated in LSC but upregulated in ITD-Flt3+ KSL cells deleted with Survivin (21 genes)symbol Name Fold change Aebp2 AE binding protein 2 (Aebp2), transcript variant 3, [NM_009637] 11. 4 Anp32b unspliced dna for: PROLIFERATION RELATED ACIDIC LEUCINE RICH PROTEIN PAL31 (SIMILAR TO ACIDIC PROTEIN RICH IN LEUCINES) homolog [Mus musculus],
2.9 App amyloid beta protein precursor, [M18373] 25.7 Cd164 CD164 antigen, [AK018009] 2.5 Crem cAMP responsive element modulator. [AK016156] 7.3 Emp1 epithelial membrane protein 1 (Emp1), [NM_010128] 2.3 Fbxl17 Mus musculus 10 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:B930094M09 product: unknown EST. [AK081162] 5.1 Gas2l3 Mus musculus 16 days embryo head cDNA, RIKEN full-length enriched library, clone:C130036O09 product: unclassifiable, [AK048137] 12.5 Myef2 myelin basic protein expression factor 2, repressor (Myef2), [NM_010852] 10.6 Nrip1 nuclear receptor interacting protein 1 (Nrip1), [NM_173440] 6.2 Pla2g12a phospholipase A2, group XIIA (Pla2g12a), [NM_023196] 3.2 Psmd11 Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone: 1700089D09 product: unclassifiable. [AK007029] 12.3 Rps20 Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830013G23 product: hypothetical protein. [AK150667] 5.5 Senp7 SUMO1/sentrin specific protease 7 (Senp7), transcript variant 3, [NM_001003972] 12.3 Slc25a16 solute carrier family 25 (mitochondrial carrier, Graves disease autoantigen), member 16 (Slc25a16), [NM_175194] 2.8 Spire1 spire homolog 1 (Drosophila) (Spire1), transcript variant 2, [NM_176832] 9.5 Tdrd3 Mus musculus adult male olfactory brain cDNA, RIKEN full-length enriched library, clone:6430599B16 product: hypothetical Tudor domain containing protein. [AK078326] 2.9 Tmpo thymopoietin (Tmpo), [NM_011605] 4.3 Trps1 Mus musculus adult male adrenal gland cDNA, RIKEN full-length enriched library, clone: 7330401C17 product: expressed sequence AI115454, [AK078617] 11.4 Ube2n ubiquitin-conjugating enzyme E2N (Ube2n), [NM_080560] 2.4 Wtap Mus musculus 12 days embryo embryonic body between diaphragm region and neck cDNA, RIKEN full-length enriched library, clone: 9430038B09 product: unclassifiable. [AK020459] 14. 2
Fold change: ITD-Flt3+ KLS cells derived from CreER-Survivinfx/fx compared to control Survivinfx/fx in two experiments. (c) Genes down regulated in LSC and in ITD-Flt3+ KSL cells deleted with Survivin (24 genes)Symbol Name Fold change Arg2 arginase type II (Arg2), [NM_009705] −2.2 Atp2c1 CALCIUM-TRANSPORTING ATPASE TYPE 2C, MEMBER 1 (EC 3.6.3.8) (ATPASE 2C1) (ATP-DEPENDENT CA2+ PUMP PMR1) homolog −2.2 Chrac1 chromatin accessibility complex 1,. [AK031914] −4.3 Dhx30 DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), [NM_133347] −3.1 Dsg2 desmoglein 2, (cDNA clone IMAGE:4036406), [BC034056] −4.1 Eef1g eukaryotic translation elongation factor 1 gamma (Eef1g), [NM_026007] −2.2 Herc5 hypothetical regulator of chromosome condensation (RCC1) containing protei
−2.3 Hmga2 high mobility group AT-hook 2 (Hmga2), [NM_178057] −5.0 Hmgb1 high mobility group box 1, [BC064790] −3.4 Hmmr hyaluronan mediated motility receptor (RHAMM) (Hmmr), [NM_013552] −3.2 Il15 interleukin 15 (Il15), [NM_008357] −6.1 Jmy junction-mediating and regulatory protein (Jmy), [NM_021310] −4.1 Lrrn1 Mus musculus 10, 11 days embryo whole body cDNA, RIKEN full-length enriched library, clone:2810047E21 product: unclassifiable, full insert sequence. [AK012914] −4.0 Maff v-maf musculoaponeurotic fibrosarcoma oncogene family, protein F (avian) (Maff), [NM_010755] −8.4 Mapk14 mitogen activated protein kinase 14 (Mapk14), [NM_011951] −2.4 Ncoa7 nuclear receptor coactivator 7, [BC076623] −8.9 Nedd1 neural precursor cell expressed, developmentally downregulated gene 1 (Nedd1), [NM_008682] −2.6 Parp11 poly (ADP-ribose) polymerase family, member 11 (Parp11), [NM_181402] −2.5 Pawr PRKC, apoptosis, WT1, regulator (Pawr), [NM_054056] −2.8 Rps4x ribosomal protein S4, X-linked (Rps4x), [NM_009094] −2.2 Smarce1 SWI [AK042961] −2.9 Tpbg trophoblast glycoprotein, [AK050794] −4.5 Vwf Von Willebrand factor homolog (Vwf), [NM_011708] −5.8 Zbtb20 zinc finger and BTB domain containing 20 (Zbtb20), [NM_019778] −3.1
Fold change: ITD-Flt3+ fx/fx compared to control Survivinfx/fx in two experiments. (d) Genes upregulated in LSC and in ITD-Flt3+ KSL cells deleted with Survivin (37 genes).symbol Name fold change Cenpa Mus musculus 0 day neonate eyeball cDNA, RIKEN full-length enriched library, clone:E130306P06 product: hypothetical protein, [AK165029] 10.7 Clcn3 chloride channel 3 (Clcn3), transcript variant c, [NM_173876] 5.5 Cpd carboxypeptidase D (Cpd), [NM_007754] 6.2 Cul4b cullin 4B,. [AK164640] 4.9 Ddx52 DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 (Ddx52), [NM_030096] 3.3 Dnajc1 DnaJ (Hsp40) homolog, subfamily C, member 11 (Dnajc11), [NM_172704] 6.4 Ephb2 Eph receptor B2 (Ephb2), [NM_010142] 3.0 Gnaq guanine nucleotide binding protein, alpha q polypeptide (Gnaq), [NM_008139] 17.2 Gripap1 premature mRNA for mKIAA1167 protein [AK173119] 2.2 Il1rap interleukin 1 receptor accessory protein (Il1rap), transcript variant 2, [NM_134103] 11.0 Itsn2 SH3 domain protein 1B, [AK161743] 4.4 Lass6 longevity assurance homolog 6 (S. cerevisiae) (Lass6), [NM_172856] 2.3 Lpp caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E. coli) (Clpp), [NM_017393] 2.1 Mppe1 metallophosphoesterase 1 (Mppe1), [NM_172630] 3.4 Mrps5 mitochondrial ribosomal protein S5, [AK047438] 9.0 Myh9 myosin heavy chain IX (Myh9), [NM_181327] 2.8 Nck2 non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), [NM_010879] 4.8 Nin microtubule associated serine/threonine kinase-like (Mastl), [NM_025979] 7.6 Nmt2 N-myristoyltransferase 2, [AK049483] 2.3 Orc5l origin recognition complex, subunit 5 homolog (S. cerevisiae), [AK054452] 4.6 Osbpl3 Mus musculus adult male aorta and vein cDNA, RIKEN full-length enriched library, clone:A530055M08 product: unknown EST, [AK040984] 8.7 Paqr3 Mus musculus adult male pituitary gland cDNA, RIKEN full-length enriched library, clone:5330440B03 product: unknown EST, [AK030634] 4.4 Pcbd2 pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 2 (Pcbd2), [NM_028281] 4.2 Rab18 RAB18, member RAS oncogene family (Rab18), [NM_181070] 6.3 Rab8b RAB8B, member RAS oncogene family (Rab8b), [NM_173413] 2.7 Rae1 RAE1 RNA export 1 homolog (S. pombe), (cDNA clone MGC:73449 IMAGE:5718934), complete cds. [BC060072] 3.3 Sfrs6 splicing factor, arginine/serine-rich 6 (Sfrs6), [NM_026499] 2.2 Sla src-like adaptor (Sla), [NM_009192] 2.7 Slc31a1 solute carrier family 31, member 1 (Slc31a1), [NM_175090] 4.2 Snx11 sorting nexin 11, [AK037747] 2.6 Sos1 Son of sevenless homolog 1 (Drosophila) (Sos1), [NM_009231] 2.4 Ssh2 mKIAA1725 protein [AK173243] 4.6 Stk3 serine/threonine kinase 3 (Ste20, yeast homolog) (Stk3), [NM_019635] 9.7 Taf1b TATA box binding protein (Tbp)-associated factor, RNA polymerase I, B (Taf1b), [NM_020614] 4.3 Vps13d PREDICTED: Mus musculus vacuolar protein sorting 13D (yeast), transcript variant 2 (Vps13d), [XM_001002241] 8.4 Wdr26 PREDICTED: Mus musculus WD repeat domain 26, transcript variant 1 (Wdr26), [XM_977731] 3.3 Xpo4 exportin 4 (Xpo4), [NM_020506] 6.9
Fold change: ITD-Flt3+ fx/fx compared to control Survivinfx/fx in two experiments.
