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Figure 2: Experimental protocol and timing of intravital TPLSM. First-round intravital TPLSM at 2 hours after inoculation. Second-round intravital TPLSM at 24 hours after inoculation in the same mouse, for imaging the early events of colorectal liver metastasis. Third-round intravital TPLSM at 8 weeks after inoculation in the same mouse, for imaging-established liver metastases and tumor angiogenesis.