In Vivo Time-Course Imaging of Tumor Angiogenesis in Colorectal Liver Metastases in the Same Living Mice Using Two-Photon Laser Scanning Microscopy

Figure 2

Experimental protocol and timing of intravital TPLSM. First-round intravital TPLSM at 2 hours after inoculation. Second-round intravital TPLSM at 24 hours after inoculation in the same mouse, for imaging the early events of colorectal liver metastasis. Third-round intravital TPLSM at 8 weeks after inoculation in the same mouse, for imaging-established liver metastases and tumor angiogenesis.