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Figure 4: Liver metastatic colonization by RFP-expressing HT29 cells. (a) Macroscopic findings. (b) Intravital TPLSM image (bar, 300 μm). (c) Microscopic hematoxylin-eosin staining (bar, 500 μm). (d) Microscopic cytokeratin 20 immunostaining (bar, 500 μm).