In Vivo Time-Course Imaging of Tumor Angiogenesis in Colorectal Liver Metastases in the Same Living Mice Using Two-Photon Laser Scanning Microscopy

Table 2

Rate of liver metastasis formation and observation by intravital TPLSM.

	RFP-HT29 cells in GFP nude mice
Liver metastases after intrasplenic inoculation	70%^a (12/17)^b at 8 weeks
Observation of metastasis by intravital TPLSM	67%^c (8/12)^d at 8 weeks

^aPercentage of liver metastases after intrasplenic inoculation.
^bThe number of mice with liver metastases/the number of mice avaiable at the indicated time points.
^cPercentage of successful observation of metastatic colonies by intravital TPLSM.
^dThe number of mice successfuly observed metastatic colonies by intravital TPLSM/the number of mice with liver metastases.