Initial Report on Differential Expression of Sprouty Proteins 1 and 2 in Human Epithelial Ovarian Cancer Cell Lines

Figure 3

The subcellular localization of Spry1 and Spry2 proteins in primary human ovarian surface epithelial cell line HOSEpiC and human epithelial ovarian cancer cell lines OVCAR-3 and SKOV-3 using confocal immunofluorescence microscopy (60X oil immersion lense). Affinity-purified antibodies were used to specifically detect Spry1 and Spry2 (green). Propidium iodide staining was performed for nuclei (red). Two top rows represent HOSEpiC cells, two middle rows show SKOV-3 cells, and the bottom rows indicate OVCAR-3 cells. As seen, the staining intensity of Spry1 and Spry2 proteins was stronger in OVCAR-3 cells compared with HOSEpiC and SKOV-3 cells. Moreover, whereas Spry2 was predominantly distributed within the whole cytoplasm in vesicular structures, Spry1 was found in both cytoplasm and nucleus. Scale bar: 50 μm.