N-Acetyltransferase 1 (NAT1) Genotype: A Risk Factor for Urinary Bladder Cancer in a Lebanese Population

Table 1

(a) PCR-RFLP primers, enzymes, and conditions for NAT1 genotyping

Detected mutation		Annealing T°C	Expected	Restriction
	Primers		fragment	digestion at
			size in bp	37°C
C^1095A	1095-F 5′-CAT TTC ACC TAT AAA AAT GTC-3′	58	132	Alw26I
	1095-r 5′-CCA AGA TAA CCA CAG GCC ATC TTT AAG A-3′
C^559T	Mt2-f 5′-ATC TCC TAG AAG ACA GCA AGT AC-3′	55	138	ScaI
	Mt2-r 5′-AGG AAC AAA ATG ATT TAC TA-3′
G^560 C	SJ-f 5′-CTC CTG CAG GTG ACC ATT GAT-3′	55	261	HinfI
	SJ-r 5′-CTT AAG AGT AAA GGA GTA GAT GAT T-3′
	1088-f 5′-CAA ACC TTT TCA AAT AAT AAT AAT AAT AAT
T^1088 A	ATT AA-3′	55	96	AseI
	1088-r 5′-GCT TTC TAG CAT AAA TCA CCA A-3′

(b) NAT1 haplotypes consensus nomenclature [19]

NAT1 haplotypes	Nucleotide change(s) and rs identifier(s)
NAT1 * 4	Reference gene sequence published in GenBank:
	accession number X17059
NAT1 * 3	1095C > A (rs15561)
NAT1 * 5	350,351G > C (rs72554606)
	497–499G > C (rs72554608)
	884A > G (rs55793712)
	Δ^976 (rs72554612)
	Δ^1105 (rs72554613)
NAT1 * 10	1088T > A (rs1057126)
	1095C > A (rs15561)
NAT1 * 14A	560G > A (rs4986782)
	1088T > A (rs1057126)
	1095C > A (rs15561)
NAT1 * 14B	560G > A (rs4986782)
NAT1 * 15	559C > T (rs5030839)
NAT1 * 16	[AAA] immediately after 1091
	1095C > A (rs15561)